Virus discovery by sequence‐independent genome amplification
Identifieur interne : 002D79 ( Main/Exploration ); précédent : 002D78; suivant : 002D80Virus discovery by sequence‐independent genome amplification
Auteurs : Helen E. Ambrose [Royaume-Uni] ; Jonathan P. Clewley [Royaume-Uni]Source :
- Reviews in Medical Virology [ 1052-9276 ] ; 2006-11.
English descriptors
- Teeft :
- Acad, Adapter, Adapter sequence, Ambrose, Ampli, Amplicons, Amplifi, Boca raton, Cation, Cdna, Cdna libraries, Cdna library, Cdna synthesis, Cell culture, Chromosomal, Clewley, Clewley figure, Clin, Clin microbiol, Clinical sample, Clinical samples, Clinical specimens, Copyright, Coronavirus, Differential display, Dnase treatment, Dsdna, Dsrna, Fication, Fragment, Genome, Genome ampli fication amplifi cation, Hinp1i, Hybridisation, Infectious diseases, John wiley sons, Lambden, Ligated, Ligation, Mboi, Microarrays, Microbiol, Mrna, Multiple displacement, Natl, Nrui site, Nucleic, Nucleic acid, Oligonucleotide, Oligonucleotides, Other methods, Parvovirus, Pathogen, Polymerase, Polymorphism, Primer, Primer extension, Proc, Proc natl acad, Random primers, Representational difference analysis, Respiratory syndrome, Restriction enzyme, Restriction enzyme sites, Restriction enzymes, Restriction fragments, Reyes, Rotavirus, Sequence data, Sequencing, Single primer, Sispa, Small amounts, Ssdna, Ssrna, Subtractive hybridisation, Tester, Torque teno virus, Unknown sequence, Unknown viruses, Vidisca, Viral, Viral genome, Viral genomes, Viral particles, Viral sequences, Virol, Virol meth, Virus, Virus discovery, Virus genome, Whole genome.
Abstract
Genome sequences from several blood borne and respiratory viruses have recently been recovered directly from clinical specimens by variants of a technique known as sequence‐independent single primer amplification. This and related methods are increasingly being used to search for the causes of diseases of presumed infectious aetiology, but for which no agent has yet been found. Other methods that do not require prior knowledge of the genome sequence of any virus that may be present in the patient specimen include whole genome amplification, random PCR and subtractive hybridisation and differential display. This review considers the development and application of these techniques. Copyright © 2006 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/rmv.515
Affiliations:
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This and related methods are increasingly being used to search for the causes of diseases of presumed infectious aetiology, but for which no agent has yet been found. Other methods that do not require prior knowledge of the genome sequence of any virus that may be present in the patient specimen include whole genome amplification, random PCR and subtractive hybridisation and differential display. This review considers the development and application of these techniques. Copyright © 2006 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Ambrose, Helen E" sort="Ambrose, Helen E" uniqKey="Ambrose H" first="Helen E." last="Ambrose">Helen E. Ambrose</name></noRegion><name sortKey="Clewley, Jonathan P" sort="Clewley, Jonathan P" uniqKey="Clewley J" first="Jonathan P." last="Clewley">Jonathan P. Clewley</name><name sortKey="Clewley, Jonathan P" sort="Clewley, Jonathan P" uniqKey="Clewley J" first="Jonathan P." last="Clewley">Jonathan P. Clewley</name><name sortKey="Clewley, Jonathan P" sort="Clewley, Jonathan P" uniqKey="Clewley J" first="Jonathan P." last="Clewley">Jonathan P. Clewley</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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